Journal:

Article Title: Expression of the EspB Protein of Enteropathogenic Escherichia coli within HeLa Cells Affects Stress Fibers and Cellular Morphology

doi:

Figure Lengend Snippet: Expression of EspB by HeLa cell clones. HeLa cells that had been transfected with vector alone or with espB were left untreated or induced for 5 days with dexamethasone as indicated. The cells were fixed, permeabilized, and stained with FITC-phalloidin to label filamentous actin (left) and with an affinity-purified antibody against EspB followed by a secondary antibody conjugated to lissamine rhodamine (right). The stained cells were examined with a laser scanning confocal microscope.

Article Snippet: All subsequent antibody treatments were performed at room temperature for 3 h. An affinity-purified anti-EspB antibody ( 30 ) was used at a dilution of 1:10 in PBS containing 0.3% BSA and detected with an anti-rabbit immunoglobulin G antibody conjugated to lissamine rhodamine B (Molecular Probes, Eugene, Oreg.) at a dilution of 1:200 in 0.3% BSA-PBS.

Techniques: Expressing, Clone Assay, Transfection, Plasmid Preparation, Staining, Affinity Purification, Microscopy